The IL-6 (Rat) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant IL-6 concentrations within any experimental sample including cell lysates, serum and plasma.
Rat IL-6 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 45HB for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
The data and subsequent graph were obtained after performing a cytokine ELISA for IL-6. Each known sample concentration was assayed in triplicate. The OmniKine™ Rat IL-6 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Rat IL-6 proteins within the range of 39-5000 pg/ml.