Description
The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and Cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the Cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarit
Reactivity
Human, Mouse
Applications
WB, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DDX3X; DBX; DDX3; ATP-dependent RNA helicase DDX3X; DEAD box protein 3; X-chromosomal; DEAD box, X isoform; Helicase-like protein 2; HLP2; DDX3Y; DBY; ATP-dependent RNA helicase DDX3Y; DEAD box protein 3, Y-chromosomal
Molecular Weight (Da)
73243/73154
Gene Name
DDX3X/DDX3Y
Protein Name
ATP-dependent RNA helicase DDX3X/Y
Human Gene ID
1654/8653
Human Swiss Prot No.
O00571/O15523
Immunogen
The antiserum was produced against synthesized peptide derived from human DDX3. AA range:14-63
Specificity
DDX3 Polyclonal Antibody detects endogenous levels of DDX3 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
