The Angiopoietin-Like 3 (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Angiopoietin-Like3 concentrations within any experimental sample including cell lysates, serum and plasma.
Catalog No
OK-0301
Format
1 plate: 12 x 8-Well Microstrips
Detection Target
Angiopoietin-Like3
Reactivity
Human
Detection Method
Colorimetric 450 nm
Assay Type
Sandwich ELISA
SwissProt ID
Q9Y5C1
NCBI Gene ID
27329
Target Synonyms
Angiopoietin-related protein 3, Angiopoietin-5, ANG-5, ANGPT5
Human Angiopoietin-Like3 recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum and/or plasma samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Angiopoietin-Like3 was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Angiopoietin-Like3. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Angiopoietin-Like 3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Angiopoietin-Like 3 proteins within the range of 32-2000 pg/ml.