The BDNF (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant BDNF concentrations within any experimental sample including cell lysates, serum and plasma.
Human BDNF recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent NM33 for serum and/or plasma samples and Assay Diluent LN28 for RPMI 10% FBS.
The data and subsequent graph were obtained after performing a cytokine ELISA for BDNF. Each known sample concentration was assayed in triplicate. The OmniKine™ Human BDNF ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human BDNF proteins within the range of 47-1500 pg/ml.