The Endostatin (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Endostatin concentrations within any experimental sample including cell lysates, serum and plasma.
Human Endostatin recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent 10WR for serum samples and Assay Diluent 1TD for RPMI 10% FBS.
Three concentrations of recombinant Human Endostatin was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Endostatin. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Endostatin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Endostatin proteins within the range of 63-4000 pg/ml.