The Progranulin (Human) OmniKine™ ELISA Kit contains the components necessary for quanititative determination of natureal or recombinant Progranulin concentrations within any experimental sample including cell lysates, serum and plasma.
Human Progranulin recombinant protein was spiked into various biological samples and serial diluted with Assay Diluent NM33 for serum samples and Assay Diluent LN28 for RPMI 10% FBS.
Three concentrations of recombinant Human Progranulin was spiked into various samples to measure the recovery percentage of recombinant protein in duplicate.
The data and subsequent graph were obtained after performing a cytokine ELISA for Progranulin. Each known sample concentration was assayed in triplicate. The OmniKine™ Human Progranulin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Progranulin proteins within the range of 63-4000 pg/ml.