}
Phospho-AMPKα (Thr172) Fluorometric Cell-Based ELISA Kit
Catalog No :
FLUO-CBP1003
Reactivity
H:T183/172,M:T183/172,R:T183/172
Assay Type
Phospho Cell-Based ELISA
Format
2 Plates: TC 96-Well Plates
Dynamic Range
At least 5000 cells/well
Size
Price/Quantity
$500
Quantity
Details
Product Name
Phospho-AMPKα (Thr172) Fluorometric Cell-Based ELISA Kit
Description
The Phospho-AMPKα (Thr172) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-AMPKα (Thr172) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-AMPKα (Thr172) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Catalog No
FLUO-CBP1003
Format
2 Plates: TC 96-Well Plates
Detection Target
AMPKα (Phospho-Thr172)
Reactivity
H:T183/172, M:T183/172, R:T183/172
Detection Method
Fluorometric
Assay Type
Phospho Cell-Based ELISA
SwissProt ID
Q13131/P54646
NCBI Gene ID
AAPK1/AAPK2
Target Synonyms
5'-AMP-activated protein kinase; catalytic alpha-1 chain; AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1; acetyl-CoA carboxylase kinase; hormone-sensitive lipase kinase; kinase AMPK-alpha1; 5'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; PRKAA2; AMPK; AMPK2
Storage and Stability Guarantee
4°C/6 Months
Sample Type
Researchers plate their cell line of choice
Range
At least 5000 cells/well
Assay Time
4.5 hours
References:
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Abstract:
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Western blot analysis of extracts from COS7 cells, treated with Adriamycin 0.5ng/ml 24h
Western blot analysis of extracts from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody.
