The Phospho-Caveolin-1 (Tyr14) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-Caveolin-1 (Tyr14) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-Caveolin-1 (Tyr14) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Western blot analysis of extracts from HuvEc cells treated with PMA 125ng/ml 30' and HeLa cells treated with LPS 100ng/ml 30', using Caveolin-1 (Phospho-Tyr14) Antibody.