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> Products > ELISA Kits > Phospho-BTK (Tyr551) Fluorometric Cell-Based ELISA Kit

Phospho-BTK (Tyr551) Fluorometric Cell-Based ELISA Kit

Overview Description
Datasheet SDS
   Intra-well Normalization      GAPDH Actin or beta Catenin controls      Ex/Em: 651/667 - 495/521   
Catalog No : FLUO-CBP1872
  • Reactivity
    H:Y551 M:Y551 R:Y552
  • Assay Type
    Phospho Cell-Based ELISA
  • Format
    2 Plates: TC 96-Well Plates
  • Dynamic Range
    At least 5000 cells/well
  • Size
    1 kit
  • Price/Quantity
    $500
  • Quantity
Add to Cart
Details
  • Product Name
    Phospho-BTK (Tyr551) Fluorometric Cell-Based ELISA Kit
  • Description
    The Phospho-BTK (Tyr551) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-BTK (Tyr708) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-BTK (Tyr708) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
  • Catalog No
    FLUO-CBP1872
  • Format
    2 Plates: TC 96-Well Plates
  • Detection Target
    BTK (Phospho-Tyr551)
  • Reactivity
    H:Y551 M:Y551 R:Y552
  • Detection Method
    Fluorometric
  • Assay Type
    Phospho Cell-Based ELISA
  • SwissProt ID
    Q06187
  • NCBI Gene ID
    BTK
  • Target Synonyms
    AGMX1; ATK; Agammaglobulinaemia tyrosine kinase; B cell progenitor kinase; BPK; Bruton's tyrosine kinase; Kinase EMB; kinase Btk
  • Storage and Stability Guarantee
    4°C/6 Months
  • Sample Type
    Researchers plate their cell line of choice
  • Range
    At least 5000 cells/well
  • Assay Time
    4.5 hours
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View Publication
Diagram 2
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Diagram 1
An illustrated breakdown of the colorimetric Non-Phospho Cell-Based ELISA assay principles.
Non-Phospho WB
Western blot analysis of lysates from HeLa cells, using BTK Antibody. The lane on the right is blocked with the synthesized peptide.
Phospho WB
Western blot analysis of extracts from HeLa cells, treated with H2O2 (100uM, 30mins), using BTK (phospho-Tyr551) antibody.

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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