The Phospho-Akt (Thr308) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-AKT2 (Thr308) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-AKT2 (Thr308) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Western blot analysis of lysates from NIH/3T3 cells, treated with PDGF 50ng/ml 20', using Akt Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells treated with Insulin, using Akt (Phospho-Thr308) Antibody. The lane on the left is 293 cells untreated.
