The Phospho-MER/SKY (Tyr749/681) Fluorometric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Phospho-MERTK (Tyr749/681) protein phosphorylation and expression profile in cells. The kit can be used for measuring the relative amounts of phosphorylated Phospho-MERTK (Tyr749/681) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals, or activators have on CAV1 phosphorylation.
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 5', using MER/SKY (Phospho-Tyr749/681) Antibody. The lane on the right is blocked with the phospho peptide.