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> Products > ELISA Kits > Phospho-PKC ε (Ser729) Colorimetric Cell-Based ELISA Kit

Phospho-PKC ε (Ser729) Colorimetric Cell-Based ELISA Kit

Overview Description
Datasheet SDS
   Crystal Violet Normalization      Customizable Format      GAPDH Actin or beta Catenin controls   
Catalog No : CBP1307
  • Reactivity
    H:S729,M:S729,R:S729
  • Assay Type
    Cell-Based ELISA
  • Format
    2 Plates: TC 96-Well Plates
  • Dynamic Range
    At least 5000 cells/well
  • Size
    1 kit
  • Price/Quantity
    $500
  • Quantity
Add to Cart
Details
  • Product Name
    Phospho-PKC ε (Ser729) Colorimetric Cell-Based ELISA Kit
  • Description
    The Phospho-PKCε(Ser729) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-PKCε(Ser729) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-PKCε(Ser729) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-PKCε(Ser729).
  • Catalog No
    CBP1307
  • Format
    2 Plates: TC 96-Well Plates
  • Detection Target
    Phospho-PKCε(Ser729)
  • Reactivity
    H:S729, M:S729, R:S729
  • Detection Method
    Colorimetric 450 nm
  • Assay Type
    Cell-Based ELISA
  • SwissProt ID
    Q02156
  • NCBI Gene ID
    5581
  • Target Synonyms
    KPCE; PKC-epsilon; PKCEA; PRKCE; Protein kinase C, epsilon type; kinase PKC-epsilon; nPKC-epsilon
  • Storage and Stability Guarantee
    4°C/6 Months
  • Sample Type
    Researchers plate their cell line of choice
  • Range
    At least 5000 cells/well
  • Assay Time
    4.5 hours
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View Publication
Diagram 2
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Diagram 1
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Cell-Based Non-Phospho Western Blot
Western blot analysis of extracts from HeLa/Jurkat/3T3 cells, treated with PMA 125ng/ml 30'
Cell-Based Phosho Western Blot
Western blot analysis of extracts from HeLa cells treated with PMA 125ng/ml 30', using PKC epsilon (Phospho-Ser729) Antibody.

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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