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> Products > ELISA Kits > Phospho-eNOS (Ser615) Colorimetric Cell-Based ELISA Kit

Phospho-eNOS (Ser615) Colorimetric Cell-Based ELISA Kit

Overview Description
Datasheet SDS
   Crystal Violet Normalization      Customizable Format      GAPDH Actin or beta Catenin controls   
Catalog No : CBP1447
  • Reactivity
    H:S615,M:S614,R:S614
  • Assay Type
    Cell-Based ELISA
  • Format
    2 Plates: TC 96-Well Plates
  • Dynamic Range
    At least 5000 cells/well
  • Size
    1 kit
  • Price/Quantity
    $500
  • Quantity
Add to Cart
Details
  • Product Name
    Phospho-eNOS (Ser615) Colorimetric Cell-Based ELISA Kit
  • Description
    The Phospho-eNOS(Ser615) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-eNOS(Ser615) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-eNOS(Ser615) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-eNOS(Ser615).
  • Catalog No
    CBP1447
  • Format
    2 Plates: TC 96-Well Plates
  • Detection Target
    Phospho-eNOS(Ser615)
  • Reactivity
    H:S615, M:S614, R:S614
  • Detection Method
    Colorimetric 450 nm
  • Assay Type
    Cell-Based ELISA
  • SwissProt ID
    P29474
  • NCBI Gene ID
    4846
  • Target Synonyms
    cNOS; Constitutive NOS; EC-NOS; ECNOS; Endothelial NOS; Nitric-oxide synthase, endothelial; NOS, type III; NOS3; NOSIII
  • Storage and Stability Guarantee
    4°C/6 Months
  • Sample Type
    Researchers plate their cell line of choice
  • Range
    At least 5000 cells/well
  • Assay Time
    4.5 hours
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Abstract:

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View Publication
Diagram 2
A short protocol of the steps taken in Assay Biotechnology's Cell-Based ELISA Kits.
Diagram 1
An illustrated breakdown of the Colorimetric Cell-Based ELISA assay principles.
Cell-Based Non-Phospho Western Blot
Western blot analysis of the lysates from HepG2 cells
Cell-Based Phosho Western Blot
Western blot analysis of extracts from K562 cells treated with EGF 40nM 30', using eNOS (Phospho-Ser615) Antibody.

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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