The Phospho-MEK1/2(Ser221) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-MEK1/2(Ser221) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-MEK1/2(Ser221) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-MEK1/2(Ser221).
Western blot analysis of extracts from K562 cells treated with serum 20% 15' and Jurkat cells treated with EGF, using MEK1/2 (Phospho-Ser221) Antibody.