The Phospho-Abl(Tyr393/412) Colorimetric Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect Phospho-Abl(Tyr393/412) protein expression profile in cells. The kit can be used for measuring the relative amounts of Phospho-Abl(Tyr393/412) in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on Phospho-Abl(Tyr393/412).
Western blot analysis of lysates from COS7 cells, treated with calyculinA 50ng/ml 30', using ABL1 Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from RAW264.7 cells, using Abl (Phospho-Tyr393/412) Antibody. The lane on the right is blocked with the phospho peptide.
