Assay Biotechnology secondary antibodies are available conjugated to enzyme, biotin or fluorophore for use in a variety of antibody-based applications including Western Blot, ImmunoHistoChemistry, ImmunoFluorescence, Flow Cytometry and ELISA. We offer high quality secondary antibodies from goat, rabbit and donkey sources for your each application. Serum adsorbed secondary antibodies are also available and are recommended for use with immunoglobulin-rich samples.
Catalog No
SA0438
Reactivity
Mouse
Applications
IF/ICC,FCM,WB
Conjugate
Dylight 800
Source
Goat
Dilution
Suggested starting 1:50-1:1000 dilutions for most fluorescent applications, while 1:5000-20000 dilutions for fluorescent assays with LI-1:COR® Odyssey equipment would be recommended.
Purification
Affinity purified using solid phase Mouse IgG (H&L) with finally > 95% purity based on SDS-PAGE
Concentration
Formulation
Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative, 1% BSA as stablizer and 50% Glycerol.
Storage and Stability
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the
original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing
To use the DyLight Fluors with fluorescent imagers, use a spectral line of the blue laser diode for DyLight 405, a cyan (488 nm) laser for DyLight 488, a green (526 nm) laser for DyLight 550 and 594, and a red (633 nm) laser for DyLight 649. The DyLight 680 and 800 fluors are compatible with laser- and filter-based infrared imaging instruments that emit in the 700 nm and 800 nm
