The protein encoded by this gene is a negative regulator of the cell cycle and was the first tumor suppressor gene found. The encoded protein also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. The active, hypophosphorylated form of the protein binds transcription factor E2F1. Defects in this gene are a cause of childhood cancer retinoblastoma (RB), bladder cancer, and osteogenic sarcoma. [provided by RefSeq, Jul 2008]
Catalog No
D12183
Reactivity
Human:K873+K874, Mouse:K866+K867, Rat:K865+K866
Applications
WB, ELISA
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
wb dilution 1:1000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
Retinoblastoma-associated protein (p105-Rb) (pRb) (Rb) (pp110)
Molecular Weight (Da)
Gene Name
RB1
Protein Name
Rb
Human Gene ID
5925
Human Swiss Prot No.
P06400
Immunogen
Synthesized Acetyl peptide derived from human Rb. at AA range: K873/872
Specificity
This antibody detects endogenous levels of Rb (Acetyl-K873/K874), It doesn't reacte with total protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western Blot analysis of 1,mouse-heart 3,mouse-brain cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)