This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]
Catalog No
A0555
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Western blot analysis of lysates from A549 cells, using PP2A-alpha (Phospho-Tyr307) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using PP2A-alpha (Phospho-Tyr307) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of Hela cell. 1,PP2A-Cα (phospho Tyr307) Polyclonal Antibody(red) was diluted at 1:200(4° overnight). HSP70 Monoclonal Antibody(3G10)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).
