SRY-box 9(SOX9) Homo sapiens The protein encoded by this gene recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal. [provided by RefSeq, Jul 2008]
Catalog No
A0576
Reactivity
Human, Mouse
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SOX9; Transcription factor SOX-9
Molecular Weight (Da)
56137
Gene Name
SOX9
Protein Name
Transcription factor SOX-9
Human Gene ID
6662
Human Swiss Prot No.
P48436
Immunogen
The antiserum was produced against synthesized peptide derived from human SOX-9 around the phosphorylation site of Ser181. AA range:147-196
Specificity
Phospho-Sox-9 (S181) Polyclonal Antibody detects endogenous levels of Sox-9 protein only when phosphorylated at S181.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with PBS 60', using SOX-9 (Phospho-Ser181) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using SOX-9 (Phospho-Ser181) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of Sox-9 (phospho Ser181) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with UV, 4° over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37° 1hour.