The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
Catalog No
A0577
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SP1; TSFP1; Transcription factor Sp1
Molecular Weight (Da)
80693
Gene Name
SP1
Protein Name
Transcription factor Sp1
Human Gene ID
6667
Human Swiss Prot No.
P08047
Immunogen
The antiserum was produced against synthesized peptide derived from human SP1 around the phosphorylation site of Thr453. AA range:421-470
Specificity
Phospho-Sp1 (T453) Polyclonal Antibody detects endogenous levels of Sp1 protein only when phosphorylated at T453.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human brain, using SP1 (Phospho-Thr453) Antibody. The picture on the right is blocked with the phospho peptide.