Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a Cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Catalog No
A0590
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein
Molecular Weight (Da)
200749
Gene Name
TSC2
Protein Name
Tuberin
Human Gene ID
7249
Human Swiss Prot No.
P49815
Immunogen
The antiserum was produced against synthesized peptide derived from human Tuberin/TSC2 around the phosphorylation site of Ser939. AA range:905-954
Specificity
Phospho-Tuberin (S939) Polyclonal Antibody detects endogenous levels of Tuberin protein only when phosphorylated at S939.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with Anisomycin 25ug/ml 30', using Tuberin/TSC2 (Phospho-Ser939) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Tuberin/TSC2 (Phospho-Ser939) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tuberin/TSC2 (Phospho-Ser939) Antibody