The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Catalog No
A0598
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
WASF1; KIAA0269; SCAR1; WAVE1; Wiskott-Aldrich syndrome protein family member 1; WASP family protein member 1; Protein WAVE-1; Verprolin homology domain-containing protein 1
Molecular Weight (Da)
61652
Gene Name
WASF1
Protein Name
Wiskott-Aldrich syndrome protein family member 1
Human Gene ID
8936
Human Swiss Prot No.
Q92558
Immunogen
The antiserum was produced against synthesized peptide derived from human WAVE1 around the phosphorylation site of Tyr125. AA range:91-140
Specificity
Phospho-WAVE1 (Y125) Polyclonal Antibody detects endogenous levels of WAVE1 protein only when phosphorylated at Y125.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells treated with Insulin 0.01U/ml 15', using WAVE1 (Phospho-Tyr125) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using WAVE1 (Phospho-Tyr125) Antibody. The picture on the right is blocked with the phospho peptide.
