Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2
Catalog No
A0800
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PRKCA; PKCA; PRKACA; Protein kinase C alpha type; PKC-A; PKC-alpha
Molecular Weight (Da)
76764
Gene Name
PRKCA
Protein Name
Protein kinase C alpha type
Human Gene ID
5578
Human Swiss Prot No.
P17252
Immunogen
The antiserum was produced against synthesized peptide derived from human PKC alpha around the phosphorylation site of Tyr657. AA range:623-672
Specificity
Phospho-PKC α (Y657) Polyclonal Antibody detects endogenous levels of PKC α protein only when phosphorylated at Y657.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COLO205 cells, using PKC alpha (Phospho-Tyr657) Antibody. The lane on the right is blocked with the phospho peptide.