cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. This subunit has been shown to interact with and suppress the transcriptional activity of the cAMP responsive element binding protein 1 (CREB1) in activ
Catalog No
A0972
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PRKAR2B; cAMP-dependent protein kinase type II-beta regulatory subunit
Molecular Weight (Da)
46346
Gene Name
PRKAR2B
Protein Name
cAMP-dependent protein kinase type II-beta regulatory subunit
Human Gene ID
5577
Human Swiss Prot No.
P31323
Immunogen
The antiserum was produced against synthesized peptide derived from human PKA-R2 beta around the phosphorylation site of Ser113. AA range:79-128
Specificity
Phospho-PKA IIβ reg (S113) Polyclonal Antibody detects endogenous levels of PKA IIβ reg protein only when phosphorylated at S113.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COS7 cells treated with PMA 125ng/ml 30', using PKA-R2 beta (Phospho-Ser113) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
