WEE1 G2 checkpoint kinase(WEE1) Homo sapiens This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from Cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]
Catalog No
A1186
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
WEE1; Wee1-like protein kinase; WEE1hu; Wee1A kinase
Molecular Weight (Da)
71597
Gene Name
WEE1
Protein Name
Wee1-like protein kinase
Human Gene ID
7465
Human Swiss Prot No.
P30291
Immunogen
The antiserum was produced against synthesized peptide derived from human WEE1 around the phosphorylation site of Ser642. AA range:597-646
Specificity
Phospho-Wee1 (S642) Polyclonal Antibody detects endogenous levels of Wee1 protein only when phosphorylated at S642.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with etoposide 25uM 60', using WEE1 (Phospho-Ser642) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using WEE1 (Phospho-Ser642) Antibody
