This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the Extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]
Catalog No
A7193
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PXN; Paxillin
Molecular Weight (Da)
64533
Gene Name
PXN
Protein Name
Paxillin
Human Gene ID
5829
Human Swiss Prot No.
P49023
Immunogen
The antiserum was produced against synthesized peptide derived from human Paxillin around the phosphorylation site of Tyr31. AA range:15-64
Specificity
Phospho-Paxillin (Y31) Polyclonal Antibody detects endogenous levels of Paxillin protein only when phosphorylated at Y31.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with TNF 200ng/ml 2', using Paxillin (Phospho-Tyr31) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Paxillin (Phospho-Tyr31) Antibody
