The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]
Catalog No
A7225
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF(paraffin section), IP, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
STAT4; Signal transducer and activator of transcription 4
Molecular Weight (Da)
85941
Gene Name
STAT4
Protein Name
Signal transducer and activator of transcription 4
Human Gene ID
6775
Human Swiss Prot No.
Q14765
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT4 around the phosphorylation site of Tyr693. AA range:660-709
Specificity
Phospho-Stat4 (Y693) Polyclonal Antibody detects endogenous levels of Stat4 protein only when phosphorylated at Y693.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT4 (Phospho-Tyr693) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT4 (Phospho-Tyr693) Antibody. The picture on the right is blocked with the phospho peptide.
