The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been
Catalog No
A7226
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), IP, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
STAT5A; STAT5; Signal transducer and activator of transcription 5A
Molecular Weight (Da)
90647
Gene Name
STAT5A
Protein Name
Signal transducer and activator of transcription 5A
Human Gene ID
6776
Human Swiss Prot No.
P42229
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT5A around the phosphorylation site of Ser780. AA range:745-794
Specificity
Phospho-Stat5a (S780) Polyclonal Antibody detects endogenous levels of Stat5a protein only when phosphorylated at S780.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT5A (Phospho-Ser780) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of Stat5a (phospho Ser780) Polyclonal Antibody, using Hela,Jurkat cell treated or untreated with PMA 125ng/ml 30', 4° over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37° 1hour.
