This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]
Catalog No
A8205
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
VDR; NR1I1; Vitamin D3 receptor; VDR; 1; 25-dihydroxyvitamin D3 receptor; Nuclear receptor subfamily 1 group I member 1
Molecular Weight (Da)
48289
Gene Name
VDR
Protein Name
Vitamin D3 receptor
Human Gene ID
7421
Human Swiss Prot No.
P11473
Immunogen
The antiserum was produced against synthesized peptide derived from human Vitamin D3 Receptor around the phosphorylation site of Ser51. AA range:16-65
Specificity
Phospho-VDR (S51) Polyclonal Antibody detects endogenous levels of VDR protein only when phosphorylated at S51.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COLO205 cells treated with Insulin 0.01U/ml 15', using Vitamin D3 Receptor (Phospho-Ser51) Antibody. The lane on the right is blocked with the phospho peptide.