This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also pla
Catalog No
A8221
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
TOP2A; TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II; alpha isozyme
Molecular Weight (Da)
174385
Gene Name
TOP2A
Protein Name
DNA topoisomerase 2-alpha
Human Gene ID
7153
Human Swiss Prot No.
P11388
Immunogen
The antiserum was produced against synthesized peptide derived from human TOP2A around the phosphorylation site of Thr1343. AA range:1311-1360
Specificity
Phospho-Topo IIα (T1343) Polyclonal Antibody detects endogenous levels of Topo IIα protein only when phosphorylated at T1343.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40nM 30', using TOP2A (Phospho-Thr1343) Antibody. The lane on the right is blocked with the phospho peptide.
