Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq, Aug 2008]
Catalog No
A8404
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PSEN1; AD3; PS1; PSNL1; Presenilin-1; PS-1; Protein S182
Molecular Weight (Da)
52668
Gene Name
PSEN1
Protein Name
Presenilin-1
Human Gene ID
5663
Human Swiss Prot No.
P49768
Immunogen
The antiserum was produced against synthesized peptide derived from human PSEN1 around the phosphorylation site of Ser357. AA range:323-372
Specificity
Phospho-Presenilin 1 (S357) Polyclonal Antibody detects endogenous levels of Presenilin 1 protein only when phosphorylated at S357.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using PSEN1 (Phospho-Ser357) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using PSEN1 (Phospho-Ser357) Antibody. The picture on the right is blocked with the phospho peptide.