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Primary Antibodies
AMPKα1/2 Antibody
AMPKα1/2 Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: B0003
Reactivity:Human,Mouse,Rat,Monkey
Applications:WB,IHC-p,IF(paraffin section),ELISA
Modification:Unmodified/Total
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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AMPKα1/2 Antibody

Western Blot analysis of COS7 cells using AMPKα1/2 Polyclonal Antibody diluted at 1:500

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Product Details
Product Name
AMPKα1/2 Antibody
Description
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Catalog No
B0003
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;
Molecular Weight (Da)
62808/62320
Gene Name
AAPK1/AAPK2
Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1/2
Human Gene ID
5562/5563
Human Swiss Prot No.
Q13131/P54646
Immunogen
The antiserum was produced against synthesized peptide derived from human AMPK alpha. AA range:140-189
Specificity
AMPKα1/2 Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (14)
A rational foundation for micheliolide-based combination strategy by targeting redox and metabolic circuit in cancer cells

Author: Jianshuang Guo, Kaihui Liu, Jiyan Wang, Hao Jiang, Mengyi Zhang, Yang Liu, Changliang Shan, Fangzhong Hu, Wenzheng Fu, Chunze Zhang, Jing Li, Yue Chen

Date of Publication : 2022-04-12

Autophagy and apoptosis mediated nano-copper-induced testicular damage

Author: Helin Chen, Yanyan Wang, Jie Luo, Min Kang, Jin Hou, Ruoping Tang, Ling Zhao, Fei Shi, Gang Ye, Xiaoli He, Hengmin Cui, Hongrui Guo, Yinglun Li, Huaqiao Tang

Date of Publication : 2021-12-15

Lactoferrin Alleviates Acute Alcoholic Liver Injury by Improving Redox-Stress Response Capacity in Female C57BL/6J Mice

Author: Deming Li, Zhiqiang Hu, Qian He, Yaxin Guo, Yu Chong, Jiaying Xu, Liqiang Qin

Date of Publication : 2021-12-07

The mitochondrial signaling peptide MOTS-c improves myocardial performance during exercise training in rats

Author: Yuan, Jinghan, Wang, Manda, Pan, Yanrong, Liang, Min, Fu, Yu, Duan, Yimei, Tang, Mi, Laher, Ismail, Li, Shunchang

Date of Publication : 2021-10-11

Metformin attenuates diabetic neuropathic pain via AMPK/NF-κB signaling pathway in dorsal root ganglion of diabetic rats

Author: Xiao-Jun Cao, Rui Wu, He-Ya Qian, Xiang Chen, Hong-Yan Zhu, Guang-Yin Xu, Ye-Zi Sun, Ping-An Zhang

Date of Publication : 2021-09-20

Bawei Chenxiang Wan Ameliorates Cardiac Hypertrophy by Activating AMPK/PPAR-α Signaling Pathway Improving Energy Metabolism

Author: Zhang Xiaoying, Zhang Zhiying, Wang Pengxiang, Han Yiwei, Liu Lijun, Li Jie, Chen Yichun, Liu Duxia, Wang Jinying, Tian Xiaoying, Zhao Qin, Yan Fengxia

Date of Publication : 2021-06-03

Autophagy was activated against the damages of placentas caused by nano-copper oral exposure

Author: Min Kang, Jie Luo, Ling Zhao, Fei Shi, Gang Ye, Xiaoli He, Suqi Hao, Dan Yang, Helin Chen, Hongrui Guo, Yinglun Li, Huaqiao Tang

Date of Publication : 2021-05-26

Gu-Ben-Fang-Xiao decoction modulates lipid metabolism by activating the AMPK pathway in asthma remission

Author: Yan-Nan You, Qiong-Qiong Xing, Xia Zhao, Jian-Jian Ji, Hua Yan, Tao Zhou, Ying-Mei Dong, Li-Shun Ren, Shu-Ting Hou, Yuan-Yuan Ding

Date of Publication : 2021-03-11

An Integrated Approach Based on Network Pharmacology Combined with Experimental Verification Reveals AMPK/PI3K/Akt Signaling is an Important Way for the Anti-Type 2 Diabetic Activity of Silkworm Excrement

Author: Huxinyue Duan, Qing Zhang, Jia Liu, Ruolan Li, Wei Peng, Chunjie Wu

Date of Publication : 2021-02-11

Outer membrane protein a in Acinetobacter baumannii induces autophagy through mTOR signalling pathways in the lung of SD rats

Author: Dan Zhao, Yumei Li, Chunhong Peng, Jieru Lin, Fuxun Yu, Yichen Zhao, Xiangyan Zhang, Degang Zhao

Date of Publication : 2020-12-31

Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways

Author: Chufan Yan, Jiangyue Zhao, Yu Qin, Fangkun Zhao, Liyang Ji, Jinsong Zhang

Date of Publication : 2020-08-01

Silver Nanoparticles Induced Oxidative Stress and Mitochondrial Injuries Mediated Autophagy in HC11 Cells Through Akt/AMPK/mTOR Pathway

Author: Jin Hou, Ling Zhao, Huaqiao Tang, Xiaoli He, Gang Ye, Fei Shi, Min Kang, Helin Chen, Yinglun Li

Date of Publication : 2020-07-14

TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways

Author: Gao, Lei, Wang, Li-you, Liu, Zhi-qiang, Jiang, Dan, Wu, Shi-yong, Guo, Yu-qian, Tao, Hong-mei, Sun, Min, You, Lin-na, Qin, Shu, Cheng, Xiao-cheng, Xie, Jun-shi, Chang, Guang-lei, Zhang, Dong-ying

Date of Publication : 2020-01-22

Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet

Author: Qing-Qing Min, Li-Qiang Qin, Zhen-Zhen Sun, Wen-Ting Zuo, Lin Zhao, Jia-Ying Xu

Date of Publication : 2018-11-02

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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