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> Products > Primary Antibodies > SGK1 Antibody

SGK1 Antibody

Overview Description
Datasheet SDS
   Kinases and Phosphatases   
Catalog No : B0087
  • Reactivity
    Human,Mouse,Rat
  • Applications
    IF/ICC,WB,IHC-p,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
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Details
  • Product Name
    SGK1 Antibody
  • Description
    This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]
  • Catalog No
    B0087
  • Reactivity
    Human, Mouse, Rat
  • Applications
    IF/ICC, WB, IHC-p, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    SGK1; SGK; Serine/threonine-protein kinase Sgk1; Serum/glucocorticoid-regulated kinase 1
  • Molecular Weight (Da)
    48942
  • Gene Name
    SGK1
  • Protein Name
    Serine/threonine-protein kinase Sgk1
  • Human Gene ID
    6446
  • Human Swiss Prot No.
    O00141
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human SGK. AA range:381-430
  • Specificity
    SGK1 Polyclonal Antibody detects endogenous levels of SGK1 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
The picture was kindly provided by our customer. Primary antibody was diluted at 1:2000. Loading control antibody was diluted at 1:20000
Application Data
Western blot analysis of lysates from Jurkat and COLO205 cells, using SGK Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using SGK Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of various cells using SGK1 Polyclonal Antibody diluted at 1:1000
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,SGK1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of human-liver tissue. 1,SGK1 Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of Hela cell. 1,SGK1 Polyclonal Antibody(red) was diluted at 1:200(4° overnight). α-SMA Monoclonal Antibody(6A12)(green) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog:SA0837 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog:SA0662 was diluted at 1:1000(room temperature, 50min).

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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