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> Products > Primary Antibodies > PKC ζ Antibody

PKC ζ Antibody

Overview Description
Datasheet SDS
   Kinases and Phosphatases   
Catalog No : B0805
  • Reactivity
    Human,Mouse,Rat,Monkey
  • Applications
    WB,IHC-p,IF/ICC,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    PKC ζ Antibody
  • Description
    Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  • Catalog No
    B0805
  • Reactivity
    Human, Mouse, Rat, Monkey
  • Applications
    WB, IHC-p, IF/ICC, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    PRKCZ; PKC2; Protein kinase C zeta type; nPKC-zeta
  • Molecular Weight (Da)
    67660
  • Gene Name
    PRKCZ
  • Protein Name
    Protein kinase C zeta type
  • Human Gene ID
    5590
  • Human Swiss Prot No.
    Q05513
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human PKC zeta. AA range:526-575
  • Specificity
    PKC ζ Polyclonal Antibody detects endogenous levels of PKC ζ protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from COS7 cells, treated with PMA 125ng/ml 30', using PKC zeta Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunofluorescence analysis of NIH/3T3 cells, using PKC zeta Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of COS7 cells using PKC ζ Polyclonal Antibody diluted at 1:2000
Application Data
Western Blot analysis of various cells using PKC ζ Polyclonal Antibody diluted at 1:2000

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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