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> Products > Primary Antibodies > Calmodulin Antibody

Calmodulin Antibody

Overview Description
Datasheet SDS
   Structural Proteins   
Catalog No : B0829
  • Reactivity
    Human,Mouse,Rat
  • Applications
    WB,IHC-p,IF/ICC,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
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Details
  • Product Name
    Calmodulin Antibody
  • Description
    This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
  • Catalog No
    B0829
  • Reactivity
    Human, Mouse, Rat
  • Applications
    WB, IHC-p, IF/ICC, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    CALM1; CALM; CAM; CAM1; CALM2; CAM2; CAMB; CALM3; CALML2; CAM3; CAMC; CAMIII; Calmodulin; CaM
  • Molecular Weight (Da)
    16838
  • Gene Name
    CALM1
  • Protein Name
    Calmodulin
  • Human Gene ID
    801/805/808
  • Human Swiss Prot No.
    P62158
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human Calmodulin. AA range:46-95
  • Specificity
    Calmodulin Polyclonal Antibody detects endogenous levels of Calmodulin protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from NIH/3T3 cells, using Calmodulin Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Calmodulin Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunofluorescence analysis of HepG2 cells, using Calmodulin Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Application Data
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Application Data
Western Blot analysis of HuvEc cells using Calmodulin Polyclonal Antibody diluted at 1:2000
Application Data
Western Blot analysis of various cells using Calmodulin Polyclonal Antibody diluted at 1:2000
Application Data
Western blot analysis of lysates from 1) HuvEc, 2) HepG2, 3) 293 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) (cat:N1292) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Application Data
Immunofluorescence analysis of Hela cell. 1,Calmodulin Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:SA0834 was diluted at 1:1000(room temperature, 50min).

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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