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> Products > Primary Antibodies > p27 Antibody

p27 Antibody

Overview Description
Datasheet SDS
   Cell Cycle Proteins   
Catalog No : B0965
  • Reactivity
    Human,Rat,Mouse
  • Applications
    WB,IHC-p,IF(paraffin section),ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
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Details
  • Product Name
    p27 Antibody
  • Description
    This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
  • Catalog No
    B0965
  • Reactivity
    Human, Rat, Mouse
  • Applications
    WB, IHC-p, IF(paraffin section), ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    CDKN1B; KIP1; Cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; p27Kip1
  • Molecular Weight (Da)
    22073
  • Gene Name
    CDKN1B
  • Protein Name
    Cyclin-dependent kinase inhibitor 1B
  • Human Gene ID
    1027
  • Human Swiss Prot No.
    P46527
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human p27 Kip1. AA range:149-198
  • Specificity
    p27 Polyclonal Antibody detects endogenous levels of p27 protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of the lysates from Jurkat cells using p27 Kip1 antibody.
Application Data
Western blot analysis of lysates from HeLa, HepG2, and COLO205 cells, using p27 Kip1 Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of COLO205 cells using p27 Polyclonal Antibody diluted at 1:500
Application Data
Western Blot analysis of various cells using p27 Polyclonal Antibody diluted at 1:500
Application Data
Immunohistochemical analysis of paraffin-embedded Human-kidney-cancer tissue. 1,p27 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,p27 Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Han, Wei, et al. "ZIC1 acts a tumor suppressor in breast cancer by targeting survivin." International journal of oncology53.3 (2018): 937-948.
Publications

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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