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> Products > Primary Antibodies > GSK3β Antibody

GSK3β Antibody

Overview Description
Datasheet SDS
   Kinases and Phosphatases   
Catalog No : B7098
  • Reactivity
    Human,Mouse,Rat
  • Applications
    IF/ICC,WB,IHC-p,IP,ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    GSK3β Antibody
  • Description
    The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
  • Catalog No
    B7098
  • Reactivity
    Human, Mouse, Rat
  • Applications
    IF/ICC, WB, IHC-p, IP, ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    GSK3B; Glycogen synthase kinase-3 beta; GSK-3 beta; Serine/threonine-protein kinase GSK3B
  • Molecular Weight (Da)
    46744
  • Gene Name
    GSK3B
  • Protein Name
    Glycogen synthase kinase-3 beta
  • Human Gene ID
    2932
  • Human Swiss Prot No.
    P49841
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human GSK3B. AA range:1-50
  • Specificity
    GSK3β Polyclonal Antibody detects endogenous levels of GSK3β protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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View Publication
Application Data
Cai, Tao, Yu Liu, and Jie Xiao. "Long noncoding RNA MALAT 1 knockdown reverses chemoresistance to temozolomide via promoting micro RNA‐101 in glioblastoma." Cancer medicine 7.4 (2018): 1404-1415.
Application Data
Western blot analysis of lysates from 1) 3T3, 2) HT29, 3) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody(2B8) (cat:N1293) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Application Data
Western blot analysis of lysates from 293, COLO205, and HT29 cells, using GSK3 beta Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using GSK3 beta Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of NIH-3T3 cells using GSK3β Polyclonal Antibody diluted at 1:2000
Application Data
Western Blot analysis of HT29 cells using GSK3β Polyclonal Antibody diluted at 1:2000
Application Data
Western Blot analysis of various cells using GSK3β Polyclonal Antibody diluted at 1:2000
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-spleen tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-brain tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-uterus-cancer tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,GSK3β Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min). Negative control was used by secondary antibody only.
Application Data
Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-spleen tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-kidney tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-kidney tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Immunofluorescence analysis of rat-lung tissue. 1,GSK3β Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Ou, Liping, et al. "Dickkopf Wnt signaling pathway inhibitor 1 regulates the differentiation of mouse embryonic stem cells in vitro and in vivo." Molecular medicine reports 13.1 (2016): 720-730.
Application Data
Jin, Xinhao, et al. "Environmental Enrichment Improves Spatial Learning and Memory in Vascular Dementia Rats with Activation of Wnt/β-Catenin Signal Pathway." Medical science monitor: international medical journal of experimental and clinical research 23 (2017): 207.
Application Data
Ma, Zhan, Shuping Lou, and Zheng Jiang. "PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway." Aging (Albany NY) 12.9 (2020): 7985.
Application Data
Ge, Lu, et al. "PRMT5 promotes epithelial‐mesenchymal transition via EGFR‐β‐catenin axis in pancreatic cancer cells." Journal of cellular and molecular medicine 24.2 (2020): 1969-1979.
Application Data
Ma, Xinqi, et al. "METTL3 attenuates proliferative vitreoretinopathy and epithelial‐mesenchymal transition of retinal pigment epithelial cells via wnt/β‐catenin pathway." Journal of Cellular and Molecular Medicine 25.9 (2021): 4220-4234.
Publications

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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