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Primary Antibodies
SIRT1 Antibody
SIRT1 Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: B8476
Reactivity:Human,Mouse
Applications:IF/ICC,WB,IHC-p,ELISA
Modification:Unmodified/Total
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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SIRT1 Antibody

Western blot analysis of lysates from 1)Mouse Ovarian granulosa cell, 2)Mouse Ovarian granulosa cell knockdown by SIRT1-siRNA, primary antibody was diluted at 1:1000, 4°over night, secondary antibody(ABT:SA0002)was diluted at 1:10000, 37° 1hour. β-Tubulin Monoclonal Antibody(5G3) (ABT:N1294) antibody was diluted at 1:5000 as loading control, 4° over night, secondary antibody(ABT:SA0001)was diluted at 1:10000, 37° 1hour.

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Product Details
Product Name
SIRT1 Antibody
Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
Catalog No
B8476
Reactivity
Human, Mouse
Applications
IF/ICC, WB, IHC-p, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SIRT1; SIR2L1; NAD-dependent protein deacetylase sirtuin-1; hSIRT1; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2
Molecular Weight (Da)
81681
Gene Name
SIRT1
Protein Name
NAD-dependent protein deacetylase sirtuin-1
Human Gene ID
23411
Human Swiss Prot No.
Q96EB6
Immunogen
The antiserum was produced against synthesized peptide derived from human SirT1. AA range:13-62
Specificity
SIRT1 Polyclonal Antibody detects endogenous levels of SIRT1 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (1)
CD38 affects the biological behavior and energy metabolism of nasopharyngeal carcinoma cells

Author: Yanshan Ge, Yuehua Long, Songshu Xiao, Lin Liang, Zhengxi He, Chunxue Yue, Xiong Wei, Yanhong Zhou

Date of Publication : 2019-02-01

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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