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> Products > Primary Antibodies > PCNA Antibody

PCNA Antibody

Overview Description
Datasheet SDS
   Cell Cycle Proteins   
Catalog No : C0298
  • Reactivity
    Human,Mouse,Rat,Monkey
  • Applications
    WB,IHC-p,IF(paraffin section),ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    PCNA Antibody
  • Description
    The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
  • Catalog No
    C0298
  • Reactivity
    Human, Mouse, Rat, Monkey
  • Applications
    WB, IHC-p, IF(paraffin section), ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    PCNA; Proliferating cell nuclear antigen; PCNA; Cyclin
  • Molecular Weight (Da)
    28769
  • Gene Name
    PCNA
  • Protein Name
    Proliferating cell nuclear antigen
  • Human Gene ID
    5111
  • Human Swiss Prot No.
    P12004
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human PCNA. AA range:61-110
  • Specificity
    PCNA Polyclonal Antibody detects endogenous levels of PCNA protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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View Publication
Application Data
Western blot analysis of lysates from HepG2 cells, using PCNA Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using PCNA Antibody. The picture on the right is blocked with the synthesized peptide.
Application Data
Western Blot analysis of COS7 cells using PCNA Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Application Data
Western Blot analysis of various cells using PCNA Polyclonal Antibody diluted at 1:2000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
Application Data
Western blot analysis of lysates from 1) HepG2, 2) HuvEC, 3) COS7 cells, （Green） primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:SA0439)was diluted at 1:10000, 37° 1hour. （Red） Tubulin β Monoclonal Antibody(5G3) (cat:N1294) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody(cat:SA0436)was diluted at 1:10000, 37° 1hour.
Application Data
Yan, Jinchuan, et al. "CD137 regulates NFATc1 expression in mouse VSMCs through TRAF6/NF-κB p65 signaling pathway." Mediators of inflammation 2015 (2015).
Publications

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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