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> Products > Primary Antibodies > EFP Antibody

EFP Antibody

Overview Description
Datasheet SDS
   Transcription Regulators   
Catalog No : C11026
  • Reactivity
    Human,Rat,Mouse
  • Applications
    WB,IHC-p,IF(paraffin section),ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    EFP Antibody
  • Description
    The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the Cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
  • Catalog No
    C11026
  • Reactivity
    Human, Rat, Mouse
  • Applications
    WB, IHC-p, IF(paraffin section), ELISA
  • Modification
    Unmodified/Total
  • Source
    Polyclonal Rabbit
  • Dilution
    Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    TRIM25; EFP; RNF147; ZNF147; E3 ubiquitin/ISG15 ligase TRIM25; Estrogen-responsive finger protein; RING finger protein 147; Tripartite motif-containing protein 25; Ubiquitin/ISG15-conjugating enzyme TRIM25; Zinc finger protein 147
  • Molecular Weight (Da)
    70989
  • Gene Name
    TRIM25
  • Protein Name
    E3 ubiquitin/ISG15 ligase TRIM25
  • Human Gene ID
    7706
  • Human Swiss Prot No.
    Q14258
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human ZNF147. AA range:201-250
  • Specificity
    EFP Polyclonal Antibody detects endogenous levels of EFP protein.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western Blot analysis of various cell lysis. Primary Antibody was diluted at 1:1000. Secondary antibody(catalogNo.:SA0439 was diluted at 1:10000
Application Data
Western blot analysis of lysates from K562 cells, using ZNF147 Antibody. The lane on the right is blocked with the synthesized peptide.
Application Data
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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