Description
This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NR1I3; CAR; Nuclear receptor subfamily 1 group I member 3; Constitutive activator of retinoid response; Constitutive active response; Constitutive androstane receptor; CAR; Orphan nuclear receptor MB67
Molecular Weight (Da)
39942
Gene Name
NR1I3
Protein Name
Nuclear receptor subfamily 1 group I member 3
Human Gene ID
9970
Human Swiss Prot No.
Q14994
Immunogen
The antiserum was produced against synthesized peptide derived from human NR1I3. AA range:71-120
Specificity
MB67 Polyclonal Antibody detects endogenous levels of MB67 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
