Description
This gene encodes a member of the protein kinase superfamily and the doublecortin family. The protein encoded by this gene contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. The microtubule-polymerizing activity of the encoded protein is independent of its protein kinase activity. Mouse studies show that the DCX gene, another family member, and this gene share function in the establishment of hippocampal organization and that their absence results in a severe epileptic phenotype and lethality, as described in human patients with lissencephaly. Multiple alterna
Reactivity
Human, Mouse
Applications
WB, IF/ICC, ELISA
Modification
Unmodified/Total
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DCLK2; DCAMKL2; DCDC3B; DCK2; Serine/threonine-protein kinase DCLK2; CaMK-like CREB regulatory kinase 2; CL2; CLICK-II; CLICK2; Doublecortin domain-containing protein 3B; Doublecortin-like and CAM kinase-like 2; Doublecortin-like kinase 2
Molecular Weight (Da)
83612
Gene Name
DCLK2
Protein Name
Serine/threonine-protein kinase DCLK2
Human Gene ID
166614
Human Swiss Prot No.
Q8N568
Immunogen
The antiserum was produced against synthesized peptide derived from human DCLK2. AA range:1-50
Specificity
DCAMKL2 Polyclonal Antibody detects endogenous levels of DCAMKL2 protein.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
