catalytic activity:ATP + a protein = ADP + a phosphoprotein. , function:Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity) . Isoform 3 is catalytically inactive. , PTM:Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation. , similarity:Belongs to the protein kinase superfamily. , similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily. , similarity:Contains 1 PH domain. , similarity:Contains 1 protein kinase domain. , subcellular location:Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane. , subunit:Interacts with TUSC4. , tissue specificity:Appears to be expressed ubiquitously. ,