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Human IgG4 (Fc) (AB3844) Rabbit mAb
Human IgG4 (Fc) (AB3844) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2415
Species Reactivity:Human
Application:WB,IF/ICC,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
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Recombinant Antibody
20 ul Size Available
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Human IgG4 (Fc) (AB3844) Rabbit mAb

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of paraffin-embedded human tonsil using Human IgG4 (Fc) Rabbit mAb (M2415) at dilution of 1:300 (40x lens).Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (SA1138) at 1:500 dilution.Blue: DAPI for nuclear staining.

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Product Details
Product Name
Human IgG4 (Fc) (AB3844) Rabbit mAb
Description
As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity (ADCC) . IgG tetramer contains two heavy chains (50 kDa) and two light chains (25 kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment) .
Catalog No
M2415
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB3844
Isotype
IgG
Application
WB, IF/ICC, ELISA
Application Dilutions
WB, 1:1000-1:5000 | IF/ICC, 1:100-1:500 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 96 - 115 of Human IgG4 (Fc)..
Specificity
This antibody detects endogenous levels of Human IgG4 (Fc) protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
IGHG4
Gene ID
Human swiss
P01861
Protein Name
Human IgG4 (Fc)
Synonyms
IGHG4; Ig gamma-4 chain C region; Human IgG4 (Fc)
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