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Myosin Light Chain 2 (Phospho Thr18) (AB4272) Rabbit mAb
Myosin Light Chain 2 (Phospho Thr18) (AB4272) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2309
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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Myosin Light Chain 2 (Phospho Thr18) (AB4272) Rabbit mAb

Western blot analysis of lysates from HeLa cells, using Phospho-Myosin Light Chain 2-T18 Rabbit mAb (M2309) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Enhanced Kit.|Exposure time: 90s.

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Product Details
Product Name
Myosin Light Chain 2 (Phospho Thr18) (AB4272) Rabbit mAb
Description
Myosin, a structural component of muscle, consists of two heavy chains and four light chains. The protein encoded by this gene is a myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. The encoded protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for this gene.
Catalog No
M2309
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T18
Clone
AB4272
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T18 of human Myosin Light Chain 2.
Specificity
The antibody detects endogenous levels of Myosin Light Chain 2 protein only when phosphorylated at Thr18. The antibody does not cross-react with Myosin Light Chain 2 phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
MYL9
Gene ID
10398
Human swiss
P24844
Protein Name
Myosin Light Chain 2 (Phospho Thr18)
Synonyms
LC20; MLC2; MRLC1; MYRL2; MLC-2C; MMIHS4; Phospho-Myosin Light Chain 2-T18
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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