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Primary Antibodies
PHD2/EGLN1 (AB4529) Rabbit mAb
PHD2/EGLN1 (AB4529) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M2136
Species Reactivity:Human
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
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Recombinant Antibody
20 ul Size Available
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PHD2/EGLN1 (AB4529) Rabbit mAb

Western blot analysis of lysates from wild type (WT) and PHD2/EGLN1 knockout (KO) 293T cells, using PHD2/EGLN1 Rabbit mAb (M2136) at 1:20000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 8s.

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Product Details
Product Name
PHD2/EGLN1 (AB4529) Rabbit mAb
Description
The protein encoded by this gene catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. HIF is a transcriptional complex that plays a central role in mammalian oxygen homeostasis. This protein functions as a cellular oxygen sensor, and under normal oxygen concentration, modification by prolyl hydroxylation is a key regulatory event that targets HIF subunits for proteasomal destruction via the von Hippel-Lindau ubiquitylation complex. Mutations in this gene are associated with erythrocytosis familial type 3 (ECYT3) .
Catalog No
M2136
Species Reactivity
Human
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB4529
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:2000-1:20000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1 - 100 of human PHD2/EGLN1.
Specificity
This antibody detects endogenous levels of PHD2/EGLN1 protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.05% proclin300.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
EGLN1
Gene ID
54583
Human swiss
Q9GZT9
Protein Name
PHD2/EGLN1
Synonyms
HPH2; PHD2; SM20; ECYT3; HALAH; HPH-2; HIFPH2; ZMYND6; C1orf12; HIF-PH2; N1
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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