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Legumain (LGMN) (AB4063) Rabbit mAb
Legumain (LGMN) (AB4063) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1414
Species Reactivity:Human,Mouse
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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Legumain (LGMN) (AB4063) Rabbit mAb

Western blot analysis of various lysates using Legumain (LGMN) Rabbit mAb (M1414) at 1:1000 dilution incubated at room temperature for 1.5 hours.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25 μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit.|Exposure time: 60s.

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Product Details
Product Name
Legumain (LGMN) (AB4063) Rabbit mAb
Description
This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.
Catalog No
M1414
Species Reactivity
Human, Mouse
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB4063
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:1000-1:2000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 210 - 302 of human Legumain (LGMN) (Q99538).
Specificity
This antibody detects endogenous levels of Legumain (LGMN) protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
LGMN
Gene ID
5641
Human swiss
Q99538
Protein Name
Legumain (LGMN)
Synonyms
AEP; LGMN1; PRSC1; Legumain (LGMN)
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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