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PAK1/2/3(Phospho Ser144/S141/S139) (AB4448) Rabbit mAb
PAK1/2/3(Phospho Ser144/S141/S139) (AB4448) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1321
Species Reactivity:Human,Mouse,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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PAK1/2/3(Phospho Ser144/S141/S139) (AB4448) Rabbit mAb

Western blot analysis of various lysates using Phospho-PAK1/2/3-S144/S141/S139 Rabbit mAb (M1321) at 1:1000 dilution.NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 1s.

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Product Details
Product Name
PAK1/2/3(Phospho Ser144/S141/S139) (AB4448) Rabbit mAb
Description
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010]
Catalog No
M1321
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Unmodified
Clone
AB4448
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around S144 of human PAK1PAK1 (Q13153).
Specificity
This antibody detects endogenous levels of PAK1/2/3(Phospho Ser144/S141/S139) protein.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
PAK1/PAK2/PAK3
Gene ID
5058; 5062; 5063
Human swiss
Q13153; Q13177; O75914
Protein Name
PAK1/2/3(Phospho Ser144/S141/S139)
Synonyms
PAKalpha; Phospho-PAK1/2/3-S144/S141/S139
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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