25% Off Select Antibodies and ELISA Kits
Sign In/Register 0

We use cookies, check our Privacy Policies.

Products
Primary Antibodies
CRK/CRKII (Phospho Tyr221) (AB3165) Rabbit mAb
CRK/CRKII (Phospho Tyr221) (AB3165) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1277
Species Reactivity:Human,Mouse,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
#
#

CRK/CRKII (Phospho Tyr221) (AB3165) Rabbit mAb

Western blot analysis of various lysates using Phospho-CRK/CRKII-Y221 Rabbit mAb (M1277) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 10s.

Delivery
Contact Us
Telephone
408-747-0185
Fax
408-747-0145
Email
order@assaybiotech.com
Promotions
Product Details
Product Name
CRK/CRKII (Phospho Tyr221) (AB3165) Rabbit mAb
Description
This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
Catalog No
M1277
Species Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho Y221
Clone
AB3165
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around Y221 of human CRK/CRKIICRK.
Specificity
The antibody detects endogenous levels of CRK/CRKII protein only when phosphorylated at Tyr221. The antibody does not cross-react with CRK/CRKII phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
CRK
Gene ID
1398
Human swiss
P46108
Protein Name
CRK/CRKII (Phospho Tyr221)
Synonyms
p38; CRKII; Phospho-CRK/CRKII-Y221
Related Products

Catalog No: SA0001

HRP* Goat Anti Mouse IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
$50

Size:
100μL

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
    © 2025 AssayBiotech. All Rights Reserved.
    Follow Us
    Terms of Use Privacy Policy