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PKC zeta (Phospho Thr560) (AB4567) Rabbit mAb
PKC zeta (Phospho Thr560) (AB4567) Rabbit mAb

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: M1258
Species Reactivity:Human,Rat
Application:WB,ELISA
Host:Rabbit
Clonality:Monoclonal
Storage and Stability:Store at-20℃. Avoid freeze / thaw cycles.

$150
# Datasheet #
# SDS #
Recombinant Antibody
20 ul Size Available
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PKC zeta (Phospho Thr560) (AB4567) Rabbit mAb

Western blot analysis of various lysates using Phospho-PKC zeta-T560 Rabbit mAb (M1258) at 1:1000 dilution.Both 293T cells and C6 cells were treated by CIP (20uL/400ul) at 37℃ for 1 hour.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (SA0002) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% BSA.|Detection: ECL Basic Kit.|Exposure time: 3s.

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Product Details
Product Name
PKC zeta (Phospho Thr560) (AB4567) Rabbit mAb
Description
Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
Catalog No
M1258
Species Reactivity
Human, Rat
Clonality
Monoclonal
Host
Rabbit
Modifcation
Phospho T560
Clone
AB4567
Isotype
IgG
Application
WB, ELISA
Application Dilutions
WB, 1:500-1:1000 | ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Immunogen
A synthetic phosphorylated peptide around T560 of human PKC zeta (Q05513).
Specificity
The antibody detects endogenous levels of PKC zeta protein only when phosphorylated at Thr560. The antibody does not cross-react with PKC zeta phosphorylated at other sites.
Formulation
The antibody is provided in liquid form in phosphate - buffered saline with 50% glycerol, 0.05% BSA, and 0.02% sodium azide.
Purification
The antibody was purified by protein A affinity chromatography.
Storage and Stability
Store at-20℃. Avoid freeze / thaw cycles.
Gene Name
PRKCZ
Gene ID
5590
Human swiss
Q05513
Protein Name
PKC zeta (Phospho Thr560)
Synonyms
PKC2; PKC-ZETA; Phospho-PKC zeta-T560
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Price/Quantity:
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Catalog No: SA0002

HRP* Goat Anti Rabbit IgG(H+L)

Price/Quantity:
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